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KMID : 0606920060140040240
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Biomolecules & Therapeutics
2006 Volume.14 No. 4 p.240 ~ p.245
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Genetic Toxicity Test of Glycidol by Ames, Micronucleus, Comet Assays and Microarray Analysis
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Kim Ji-H.
Kim Ki-Y.
Kwon kyoung-J.
Go Seo-Y.
Min Kyung-N.
Lee Woo-S.
Park Sue-N.
Sheen Yhun-Y.
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Abstract
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The primary use for glycidol is as a stabilizer in the manufacture of vinylpolymers, however, it is also used as an intermediate in the production of pharmaceuticals, as an additives for oil and synthetic hydraulic fluids, and as a diluting agent is same epoxy resins. In this study, we have carried out in vitro genetic toxicity test of gly- cidol and microarray analysis of differentially expresed genes in response to glycidol. The result of Ames test showed mutations with glycidol treatment in base substitution strain TA1535 both with and without exogenous metabolic activation. Likewise, glycidol showed mutations in frame shift TA98 both with and without exogenous metabolic activation. The result of COMET assay in L5178Y cells with glycidol treatment showed DNA damage both with and without exogenous metabolic activation. Glycidol increased micronuclei in CHO cells both with and without exogenous metabolic activation. 150 Genes were selected as differentially eepressed genes in reponse toglycidol by microarray analysis and these genes would be candidate biomarkers of genetis toxic action of glycidol.
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KEYWORD
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Glycidol, Ames test, COMET assay, MN assay, Microarray, S9 fraction
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